ウォーターズ社・James I. Langridge博士によるセミナーを開催いたします。質量分析イメージングの新しい技術についてご紹介いただきます。ふるってご参加ください。
Advances in Multi-modal MS imaging
Dr. James I. Langridge
Waters MS Technologies Centre, Wilmslow, UK.
Desorption electrospray ionisation (DESI) is an established technique in the field of mass spectrometry, and has over recent years been used for tissue imaging (MSI). DESI results for lipids and small molecules can be of high quality compared to the more established matrix assisted laser desorption ionisation (MALDI) imaging technique. An additional advantage of DESI is that it can be performed without further sample preparation, such as matrix deposition. Using optimized gas and solvent flow rates, the DESI technique can be non-destructive and doesn’t destroy the tissue surface.
Multi modal imaging is a concept that combines the strengths of different imaging modalities to provide a more complete picture of the biological question. This can involve the use of morphological imaging such as H&E staining, functional imaging like Positron Emission Tomography (PET) or the use of molecular imaging like Mass Spectrometry Imaging (MSI). There can be significant benefit of combining or fusing these technologies.
Within the scope of MSI, different ionisation techniques have been demonstrated to have complementary strengths. Here we will describe technology improvements made to both matrix assisted laser desorption ionization (MALDI) and desorption electrospray ionization (DESI), and demonstrate the benefits of combining the two approaches with examples in the pharmaceutical DMPK area and imaging in the pre-clinical and clinical application space.